Product Name :
mAID tag Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
Function :
Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
Synthetic peptide immune sequence is PKDPAKPPAKAQVVGWPC.
Species reactivity:
Species independent
Verify application:
WB
Coupling :
unconjugated
Clone number:
RRID:
AB_3069193
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃.5-Bromobenzene-1,3-diamine Chemscene Avoid repeated freeze / thaw cycles.5-Bromopyridine-2-carbaldehyde site
Storage buffer solution:
1*PBS (pH7.PMID:33417162 4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Immunogen affinity purified.
ELTSA :
Molecular weight :
Positive control :
Mini-AID-tagged fusion protein.